Are there specific media requirements when using mRNA for transfection? Also, would I have to change the media after transfection?

Answer

No, there are no specific media requirements or restrictions when using mRNA for transfection. The only recommendation is to ensure that there is no serum or antibiotics present during the complexation process of the lipid and mRNA within the transfection protocol. We use OptiMEMĀ® I Reduced Serum Medium in our transfection protocol.

The delivery of mRNA does not require a media change after transfection. Our common practice is to not change media within the first 24-hours following transfection so as to minimize handling of cells.