Is transfection with mRNA faster? What are standard incubation times?

Answer

Yes, transfection with mRNA results in faster and more immediate translation of protein and therefore, faster expression. We visually see expression of GFP in some cell lines as fast as 90-minutes after transfection. Additionally, transfection of mRNA with Lipofectamine® MessengerMAX™ also provides prolonged duration of expression (GFP expression lasting for 5-days post-transfection), due to its ability to protect mRNA from degradation during transfection.