Do you have any technology that allows you to culture in the incubator and completely eliminate the water tray and eliminate microbe-supporting humidity during your cell culture? What can you do to eliminate transfers for centrifugation steps and for cryopreservation into cryovials? Do you have any cell culture technology that eliminates transfers by spinning directly and cryopreserving directly in the original cell culture container?

Question

Do you have any technology that allows you to culture in the incubator and completely eliminate the water tray and eliminate microbe-supporting humidity during your cell culture? What can you do to eliminate transfers for centrifugation steps and for cryopreservation into cryovials?  Do you have any cell culture technology that eliminates transfers by spinning directly and cryopreserving directly in the original cell culture container?

Accepted Answer

These questions are outside of the general cell contamination scope of this forum but we are happy to help you. These technology specific questions would be better answered by a Corning specialist who could find a tailored process based solution for you. Please contact one of the following regional Scientific Support groups and one of our agents can help you or put you in contact with a Field Applications Specialist. Americas: Scientificsupport@corning.com, Greater Asia (including Greater China, Southeast Asia, Korea, India, Australia and New Zealand):ScientificSupportAP@corning.com, EMEA (including Europe, Middle East and Africa): ScientificSupportEMEA@corning.com, Japan: ScientificSupportJP@corning.com

Answer

We had eliminated the use of antibiotics in our lab, but after a recent contamination, our new lab manager recommended we start using antibiotics for some of our more long term cultures. Do you think this is a good idea?

We want to test our cultures for sterility but there doesn’t seem to be an easy way to do this. Are you aware of any rapid tests for sterility like you see for endotoxin?

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