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Although DMSO is an extensively applied cryoprotectant, there are still some disadvantages of this compound, such as neurological toxicity and genotoxicity. In cases of tissue freezing, researchers would like to avoid DMSO as the cryoprotectant, especially for downstream clinical applications. The selection of alternative compounds varies from tissue origin and the following application. Many DMSO alternatives have been proven on tissue freezing, including non-penetrating cryoprotectants (e.g., glucose, sucrose, galactose, or trehalose) and intracellular cryoprotectants (e.g., ethylene glycol, propylene glycol, glycerol, formamide, methanol, and butanediol). Some commercial products are also available in the market. You may find more details on the following references.