Do you know of any place where there are protocols for creating different types of organoids based on cell types?


In recent years, Corning® Matrigel® matrix has developed into a powerful new tool for organoid cultures in areas like basic research and drug discovery, with several protocols available to support this work. Please find below some technical document suggestions that support the creation of different types of organoids.

Citations on Corning® Matrigel® Matrix and Organoid Culture
A compilation of research article citations, where several organoid types are referenced.

Modeling Development and Disease with Organoids

In this review article, Hans Clevers gives an overview on several organoid types and how they are generated. Using this foundational information as a starting point, we would recommend conducting a specific literature search on specific organoid types to find the most appropriate protocol for the desired application.

Organoid Models and Applications

Visit the Corning Life Sciences organoid models and application web page to learn more about a variety of organoid types and to access relevant published landmark papers that discuss methodologies to generate organoids.

For more specific organoid resources:

Gastrointestinal organoids

Single Lgr5 Stem Cells Build Crypt–villus Structures in vitro Without a Mesenchymal Niche

Cerebral organoids

Cerebral Organoids Model Human Brain Development and Microcephaly

Prostate organoids
Identification of Multipotent Luminal Progenitor Cells in Human Prostate Organoid Cultures

Kidney Organoids

Directing Human Embryonic Stem Cell Differentiation Towards a Renal Lineage Generates a Self-Organizing Kidney

Ovarian organoids

Long-term organoid culture reveals enrichment of organoid-forming epithelial cells in the fimbrial portion of mouse fallopian tube

Liver and pancreas organoids
Culture and Establishment of Self-renewing Human and Mouse Adult Liver and Pancreas 3D Organoids and their Genetic Manipulation

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