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Generating organoids is a delicate procedure and requires adjustments and testing for the specific cells and applications used. Culturing organoids is a likewise sensitive process and the specific requirements of the cells and the organoids needs to be evaluated.
The successful generation and long-term culture of organoids depends on many factors, including:
- Cell type (stem cells? From which organ?)
- Culture conditions (culture conditions, medium, ECMs used ?)
- Cell challenges (Low viability, organoids that disassemble, etc.)
- What assay is planned after the generation of the organoids?
Since this information is not outlined in this question, we wanted to share some helpful technical documents on the generation and culture of organoids for a period longer than 24 hours:
- A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening
- Culture of Mouse Intestinal Organoids in Corning® Matrigel® Matrix for Organoid Culture
- High Throughput Gene Expression Analysis of 3D Airway Organoids