Do you recommend sequencing plasmid before using?
This question is part of the following Ask The Expert session:
Ask the Expert – Transfection Best Practices for AAV Gene Therapy
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Answer
Yes, we recommend sequencing plasmids before use. Confirming the sequence and identify of plasmids could ultimately save time and resources spent troubleshooting a transfection process that might not ever yield infectious viral particles.
This post is sponsored by Corning.