I am growing intestinal organoid from iPSC cells in Corning Matrigel matrix. I plan to collect the organoids from the matrix for subsequent tests and analysis. Can you please recommend a protocol for harvesting?
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Going tiny is the next BIG thing: Tools and Techniques for Organoid Cultures
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We recommend Corning® Cell Recovery Solution, 100mL (Product #354253) for harvesting organoids from Corning Matrigel matrix. Below is a general guideline, using an example of six week old organoids in 96-well spheroid microplates. Incubation time may be optimized for best results.
- Wash wells twice with 150 µL cold PBS (organoids can be settled by gravity or centrifugation)
- Add 150 µl of cold Corning® Cell Recovery Solution per well of 96-well spheroid microplate
- Incubate at 4°C for one hour
- Wash organoids 1x with 150 µL cold PBS
- Re-suspend organoids in fresh medium or desired buffer for subsequent analysis
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