What are your recommendations for culture vessels that work best with organoids? Something that works well with imaging too.

Answer

The type of culture vessels used for organoid culture is dependent on the protocol being used for organoids. Different culture vessels are used at the various stages of generation, culture and characterization of organoids. Tissue culture-treated vessels (multi-well plates or dishes) can be used together with a natural (Corning Matrigel matrix, collagen, laminin/entactin) or a synthetic hydrogel, to initiate the organoid formation and culture in static culture. Long-term organoids cultures have also been performed in an agitated culture system like an orbital shaker or spinner flasks (Lancaster and Knoblich, Nat Protoc, 2014). Corning spheroid microplates can also be used to generate and culture organoids.  Organoids have also been cultured in drops of a culture medium, either hanging from a plate sustained by gravity and surface tension or in Corning low attachment plates. (Hohwieler M, et al. Gut, 2017, Shamir and Ewald, Nat Rev Mol Cell Biol, 2014).

In terms of imaging, Corning provides a number of microplates in 96 & 384-well formats that are compatible with high-throughput imaging technologies.