Have you seen differences in the data you generate using automation vs. manually? Can you explain?
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How to get the most out of automating your ELISA-like assays, dos and don’ts
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Job Title: Senior Researcher for the High Throughput Antibody Discovery team
Answer
I don’t generally see differences in assay data in a manual vs. automated comparison. What shows up positive in an automated assay should show up positive in a manual assay, so to speak. The differences that we see are usually in the quality of the data. Replicates on a plate show lower %CV in an automated workflow when compared to a manual workflow, as well as replicates across plates. This is assuming that liquid handling methods have been properly optimized. A poorly optimized liquid handling protocol can, and usually will, produce very poor quality data.
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