How can you ensure that your media choice won’t interfere with your imaging? Are there certain components to avoid?
This question is part of the following Ask The Expert session:
Fluorescent Cell Testing Measures – Assessing cell proliferation and cell health using flow cytometry and imaging platforms
Company: Thermo Fisher Scientific, Inc.
Job Title: R&D Associate Director
Phenol Red can cause problems with imaging, it is best to use a media that does not have phenol red in the formulation. One challenge with live cell fluorescence imaging is in imaging weak fluorophors without causing cell damage, photobleaching, or changes to cell health. A newer media from Gibco is FluoroBrite DMEM; it is a DMEM-based formulation with background fluorescence that is comparable to PBS and much lower than that from standard phenol red–free DMEM. FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophors to enable detection of weak fluorescent events in an environment that promotes optimum cell health.