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Emerging iPSC colonies do not typically have the same morphologies as established iPSCs. Pluripotent Marker expression can aid in determining pluripotency. Alkaline Phosphatase activity is one of the first markers that is highly expressed during reprogramming. We recommend using the Alkaline Phosphatase Live Stain. This allows you to monitor AP activity via a transient fluorescent signal which is non-destructive in nature. Its use on the master reprogramming plate will not compromise your cells and can be used again and again throughout the process.
See sample data using the Alkaline Phosphatase Live Stain