My iPSC have demonstrated their self-renewal and differentiation potential, but how do I know they measure up to other established ESCs or iPSCs?

Answer

An alternative to looking at marker expression via antibody staining is to look at transcription of genes associated with self-renewal and differentiation potential. The Taqman® hPSC Scorecard™ Assay allows you to determine this through a panel of self-renewal and pluripotency markers. The panel enables qPCR analysis of either undifferentiated or differentiated cells with a single assay. It can tell you whether your differentiated iPSC have the hallmark self-renewal gene expression profile and whether your differentiated samples have tri-lineage potential or skew towards particular lineages. In addition, the cloud-based software compares your results against a panel of established reference cell lines.

Learn more about the hPSC Scorecard assay and software