I am looking for a good resource for using CRISPR in CHO genome editing. We are just beginning to look at this technology for improving cell characteristics. Any advice?
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Here is a website with background information on using CRISPR for genome editing.
http://www.lifetechnologies.com/us/en/home/life-science/genome-editing/geneart-crispr/crispr-cas9-based-genome-editing.html .
CRISPR-Cas systems have become powerful tools for genome editing and the system as a good choice for your needs. This website also has a short video that will probably be helpful. The CRISPR Nuclease vector is the system for mammalian cells if you don’t have any promoter constraints.