I am trying to culture human iPSCs without a feeder layer and keep them undifferentiated. I am using a commercial iPSCs media. I am trying to do this without matrigel, but the cells keep differentiating. Do I need to add Matrigel or is there a way to do this without?

Answer

(A)iPSC feeder free cultures would primarily require a matrix ( substrate) and robust culture media systems to enable and sustain stem cell state and at the same time allow expansion of the iPSC within the culture system. Without a substrate and culture media system, the cells will crash. You could consider using alternative substrates including Geltrex®, CellStart™ or Vitronectin as part of your culture system. These products are manufactured in GMP conditions and the reproducibility of performance is well validated. The only way PSCs could be grown without a substrate/matrix would be to grow them as small aggregates in suspension cultures.

Learn more about 3D matrices for stem cell research – http://www.lifetechnologies.com/us/en/home/life-science/stem-cell-research/induced-pluripotent-stem-cells/extracellular-matrices-psc-culture.html