This question is part of the following Ask The Expert session:
iPSC quality of the starter population and seeding densities are critical for cardiac differentiation depending on the protocol you are adopting for your differentiation to cardiomyocytes. If you have expanding populations in the beginning during differentiation, the stochastic ratios alter and could lead to less robust methods of generating desired cell types. Similarly if the starting quality of iPSCs is poor, it will eventually result in poor differentiation of desired lineages.
One product you could try to improve your efficiency is the new PSC Cardiomyocyte Differentiation kit offered by Life Technologies. This kit offers a simple and reproducible three-step protocol to generate cardiomyocytes. Early cardiomyocytes can be generated as early as eight days and can be matured for the length of time required. Cells differentiated using this kit are applicable to studies including cardiotox screening, cardiac development, disease modeling, etc.
Learn more about the PSC Cardiomyocyte Differentiation Kit – http://www.lifetechnologies.com/us/en/home/life-science/stem-cell-research/stem-cell-differentiation/culture-systems-reagents-cardiomyocyte-differentiation.html