I am wondering how the assay transfer works. How difficult is it to transfer and how do you validate after automation?
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How to get the most out of automating your ELISA-like assays, dos and don’ts
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Job Title: Senior Researcher for the High Throughput Antibody Discovery team
Answer
The term “assay transfer”, in this context, simply means moving an assay from the bench (manually completed) to being run on some form of automation. This assumes that the assay has already been properly developed and validated, if appropriate. Ideally, when performing an assay transfer to an automated platform you would compare the values of your control samples, be it EC/IC50, Signal-to-Noise ratio or some other quantitative measure. They are run in a manual assay and in an automated assay. If everything has been done correctly those values should be equivalent, within a reasonable %CV.
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