I have cells expressing GFP and I want to use CSFE, but they both are green-emitting. Can you suggest something?
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Fluorescent Cell Testing Measures – Assessing cell proliferation and cell health using flow cytometry and imaging platforms
Company: Thermo Fisher Scientific, Inc.
Job Title: R&D Associate Director
There is another popular dye, CellTrace Violet, which uses violet excitation with blue emission. CellTrace Violet is fully compatible with GFP. CellTrace Violet and CellTrace CSFE are both amine-reactive succinimidyl ester compounds that covalently bind to proteins and give a bright homogenous fluorescence. When cells divide, the fluorescent label is distributed equally between daughter cells. As a result, each successive generation in a population of proliferating cells gives a reduction of fluorescence intensity, and generations can be identified.