I have heard that certain editing methods work better with certain cell lines, if so how do you know which method to use with your cell line?
This question is part of the following Ask The Expert session:
Choosing the Right Genome Editing Technology for your Application and Research
Company: Thermo Fisher Scientific, Inc.
Job Title: R&D Lead
The key is to know the best format of nucleic acid and the delivery reagent that is ideal for your cell type. For most cell lines we have tested complete CRISPR RNa format (Cas9 mRNA + in vitro transcribed gRNa) with Lipofectamine messenger max works well. Please note certain cell types electroporation works better. Refer to tranfection reagent guidelines (http://www.lifetechnologies.com/us/en/home/life-science/cell-culture/transfection/transfection-support.html)