In thinking about moving a lenti process from adherent to suspension, how do you manage shear concerns?
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Company: MilliporeSigma
Job Title: Head of Technical Operations, Viral and Gene Therapy Manufacturing
Answer
It comes down to selecting a cell line and media combination that has been adapted for growing under suspension conditions. This may take the cloning and screening of some several hundred clones followed by screen such clones for growth and productivity attributes. Typically one adds reagents such as Pluronic F-68 to the media to minimize shear, but one has to balance the effect of PF68 as such can affect the transfection capability when using an all plasmid production approach. Our VirusExpress cell line and media was developed to grow well under suspension conditions from small scale shaker flasks to large scale bioreactors.
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