Organoid culture is dependent on matrices/scaffolds, typically Corning® Matrigel® matrix, which itself can provide many growth factors, etc. that contributes to organoid growth/differentiation. Are there alternative, less bioactive matrices that can support organoid culture?

Answer

At Hubrecht Organoid Technology, Corning Matrigel matrix has been by far the best extracellular matrix (ECM) supporting the expansion of the organoid models. To reduce variability generated by the presence of grow factors or other proteins/molecules that could impact organoid growth, we always work with growth factor-reduced and phenol red free versions of Matrigel matrix. We have looked for a synthetic alternative to biological extracellular matrices, but to date nothing we have tested supports the growth of organoids to a comparable level as Matrigel matrix.

To support the reproducibility and consistency essential for organoid research, Corning recently launched Matrigel matrix for organoid culture. This optimized formulation is verified to support growth and differentiation of organoids1 with each lot measured for matrix stiffness, a property that supports organoid workflow. Matrigel matrix for organoid culture has been demonstrated to successfully grow organoids from both healthy and diseased cell origins2. Each lot is qualified to form stable “3D dome” structures commonly used in organoid culture.