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This question is particularly difficult to answer as there are no “standard” 2D or 3D cell culture protocols in which the format, volumes and protocols are comparable. In general, cell culture products used for 2D are less advanced than products offered for 3D cell culture and therefore, the direct cost for 3D cell culture are typically more than for an experiment with a conventional monolayer culture. Additionally, within a few days, a simple monolayer of cells can be formed; the generation of 3D cell structures like organoids might take up to several days, but during this period no elaborate steps need to be conducted by the cell culture personnel.
However, there are certain applications where conventional 2D experiments will not lead to the same rich answers as experiments conducted in 3D cell culture, as cells grown in 3D more closely mimic in vivo behavior in tissues and organs. 3D cell culture environments create more biologically relevant models for drug discovery which may lead to more predictive results, higher success rates for drug compound testing, a faster path to market, and reduced development costs. If you are interested in setting up 3D cell culture and the generation of organoids in your lab, products and solutions are available that make starting with 3D cell culture easy, e.g. Corning spheroid plates or Corning Matrigel matrix for organoid culture.
Other resources on 3D Cell Culture:
- All About Organoids e-book
- Explore Organoid Model Environments
- Citations on Corning® Matrigel® Matrix and Organoid Culture
- Working Successfully with Organoids
- Explore Spheroid Model Environments
- Citations on Corning Spheroid Microplates with Ultra-Low Attachment Surface
- Spheroid Culture Advancements