Our lab has isolated mesenchymal stem cells from tissue and we have shown differentiation into adipose, osteo and condro, but we still need to demonstrate their multipotency using phenotypic characterization. Do you have any suggestions on the best way to do this? Thanks

Answer

As per the minimal criteria defined by ISCT in 2006, in addition to in vitro differentiation of multipotent mesenchymal stromal cells (MSC) into adipocytes, osteoblasts and chondroblasts, cells must adhere to plastic and show positive expression of CD105, CD73, CD90 and absence of CD45, CD34, CD14, CD19 and HLA-DR {Dominici et al., Cytotherapy (2006) 8, 315-317}. Phenotypic characterization can be carried out via flow cytometric analysis of antibody stained cells.