My iPSC clones appear to demonstrate the markers of self-renewal, is this enough to confirm pluripotency?


No, self-renewal is a key measure of pluripotency, but confirmation of differentiation potential is also required. The use of in vitro differentiation via EB formation allows the iPSC to spontaneously differentiate into cells that are characteristic of the three germ layers. These differentiated cultures can be probed with antibodies against SMA, beta 3 Tubulin, and AFP for mesoderm, ectoderm, and endoderm respectively.

For a complete list of PSC and tri-lineage markers, please visit

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