Do you have a recommendation for concentration level of poloxamer in the media, is it different for different cell lines and is there any reason to optimize?

Answer

The typical concentration in chemically defined cell culture media is usually 1-2g/L but this is always dependent on the cell line, the media composition, the process, etc. We also had some applications where we could reduce the concentration to 0,5g/L thanks to our improved Poloxamer 188 and still had full shear protection in our model system and with our cell line. It may be worth trying to reduce Poloxamer concentration as it contributes to foaming and impacts oxygen transfer. However, the main function in the media is the shear protection effect and this should not be put at risk.