We are transitioning from feeder to feeder free culture. Can you recommend a method for this process?


Instructions for transitioning to Essential 8® Medium can be found here: http://www.lifetechnologies.com/us/en/home/references/protocols/cell-culture/stem-cell-protocols/ipsc-protocols/culturing-puripotent-stem-cells-essential-8-medium.html. To minimize the stress associated with the transfer process we generally will follow the guidance under the “Adaptation using Geltrex® LDEV-Free, hESC-Qualified Basement Membrane Matrix as an Intermediary” section. Subsequently, cells can be passaged using EDTA (i.e., Versene Solution, Cat #15040-066) and transitioned over onto truncated recombinant vitronectin (Cat # A14700) surface if desiring a leaner matrix.