We use our own homemade medium that is based on the recipe published by Toshiro Sato and Hans Clevers (Sato et al., 2009). What is different about the IntestiCult medium – what’s in it?

Answer

IntestiCult™ Organoid Growth Medium (Mouse) is an optimized version of the published medium (1), and has been developed in collaboration with Hans Clevers and The HUB Foundation for Organoid Technology. STEMCELL Technologies is the exclusive manufacturer and worldwide distributor of this complete, defined and serum-free medium for mouse intestinal organoids. The optimized recipe allows for less variability and standardized experiments as conditioned media is not required.

(1) Sato T, et al. Nature 459: 262-265, 2009