What are the minimum protein concentrations required for Matrigel matrix to form a gel?
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The minimum protein concentration may be application dependent. You should use the lot specific protein concentration from the Certificate of Analysis to determine the optimal protein concentration range for your specific application. In general, Matrigel matrix diluted to a concentration of 3 mg/mL will form a firm gel. For in vivo applications do not dilute Matrigel to a final concentration below 4 mg/mL.