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This is highly dependent on the cell type that you are using for experiments. Some types of cells require higher MOI than others, and some cell types are very sensitive to high MOI’s. You need measure your LV titer, and when optimizing your experiment, try a range of MOI’s and measure protein transduction to determine your optimal level for your specific experimental set up. To increase transduction efficiency, you can add 8ug/ml of Polybrene, associated with a 30 minute room temperature spin (2000 rpm) to increase transduction efficiency.