What method do you think is the best for ease of use for transient transfection in HEK293?
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HEK293 cells are highly transfectable. The cells can grow as both adherent if using medium with serum or they can grow in suspension is using a serum free medium. If your cells are adherent my suggestion is to use Lipofectamine 2000, if you are growing your 293 cells in suspension FreeStyle, 293fectin are good choices.
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