What method do you think is the best for ease of use for transient transfection in HEK293?


HEK293 cells are highly transfectable. The cells can grow as both adherent if using medium with serum or they can grow in suspension is using a serum free medium. If your cells are adherent my suggestion is to use Lipofectamine 2000, if you are growing your 293 cells in suspension FreeStyle, 293fectin are good choices.

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