What’s the advantage of knock out by TAL or CRISPR compared to vector-based stable shRNA?
This question is part of the following Ask The Expert session:
Choosing the Right Genome Editing Technology for your Application and Research
Answered by:
Answer
TAL and CRISPR edits the genome and hence more efficient. In case of stable shrNA the expressed shRNA works at the level of transcripts hence the effect of knock down in gene expression depends on the level of expression of shRNa the activity of the promoter at the locus where the shRNa is stably integrated as well as the ratio of shRNa to mRNa transcripts.