Ultrafiltration for Protein Concentration – Centrifuge and Crossflow Solutions

We recently finished our Ask the Expert discussion on “Laboratory based therapeutic and diagnostic marker protein concentration through centrifugal and crossflow ultrafiltration”. Ultrafiltration is an important tool in concentrating therapeutic and diagnostic marker proteins. Efficacy of ultrafiltration processes can be very application dependent and thus may raise technical questions in process planning. Two of the primary methods are using centrifugation or crossflow ultrafiltration. When considering the ultrafiltration method that is best for your application, it is critical that you select the appropriate method for target recovery and sample volume, while still considering speed and efficiency.

During this Ask the Expert session, we covered topics related to ultrafiltration of large sample volume concentration, sensitive proteins and protein degradation, when to use cassettes vs. centrifuge, viral vector concentration, membrane selection and non-protein concentrations.

This Ask the Expert session was hosted by Rik McRae, Technical Manager for Operations, Sartorius Stedim Lab, Ltd. as our expert. Rick has over 20 years of experience in product and application research and development, production engineering and technical guidance and is currently a member of the Sartorius lab ultrafiltration team. Rik is a subject matter expert for technical support and application knowledge, with strong expertise in therapeutic protein, drug delivery nanoparticle, and diagnostic marker concentration applications, with centrifugal, pressure driven and lab scale tangential flow filtration systems.

Please see ““Laboratory based therapeutic and diagnostic marker protein concentration through centrifugal and crossflow ultrafiltration” for a full transcript of the discussion: