Ask the Expert with Dr. Nirupama Shevde – Pluripotent Stem Cell Culture

By on April 16, 2014
Ask the Expert

Our Next Session – Pluripotent Stem Cell Culture

Pluripotent stem cell (PSC) culture has evolved over the past decade, just as stem cell researcher needs have been changing and the market has been growing. Initial culture conditions for stem cells used FBS and mouse embryonic feeders (MEFs). The need for a more defined system brought about the introduction of KnockOut™ Serum Replacement (KSR) in 1997 for the culture of mouse embryonic stem cells (ESCs). Even today, KSR has remained an integral part of the feeder based workflow for both mouse and human PSC culture. The desire to move away from the variability and work required to maintain MEFs led to the development of feeder-free media systems. There are a number of options for feeder-free based media and matrices; StemPro® hESC SFM and Essential 8™ media are two robust options available and both work with Geltrex® and Vitronectin matrices. As choice of media systems for PSCs grows, understanding which media to use for a specific application becomes an important consideration. Contact Life Technologies about stem cell products and services –

Join Dr. Nirupama (Rupa) Shevde, customer training manager at Thermo Fisher Scientific, to ask your questions regarding the culture of pluripotent stem cells. Questions can range from best culture options and technologies for deriving pluripotent stem cells to how to adapt cells to a new media and everything in between.

Dr. Nirupama (Rupa) Shevde is the Customer Training Manager in the Primary and Stem Cell Systems group at Life Technologies. She obtained her doctorate at Harvard University and her postdoctoral training at USCD and Ligand Pharmaceuticals in San Diego, CA. She has extensive experience in both mouse and human pluripotent stem cell research and served as a Senior Scientist and Director of Education at WiCell Research Institute and Morgridge Institute for Research since 2005 until July 2012. Under the scientific leadership of Dr. James Thomson, Rupa developed the Stem Cell Training Course which has served over 800 scientists from 32 US states and 20 countries. Most recently, Rupa was able to work in Dr. Thomson’s laboratory and gain expertise in the novel Essential 8™ Medium, Vitronectin (VTN-N) substrate and non-integrating episomal reprogramming technology developed by Dr. James Thomson and marketed by Life Technologies.

Don’t miss this chance to have your pluripotent stem cell culture questions answered starting Monday April 21st!Its magic mug

First 5 submittals get a free Magic Science mug from Life Technologies


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One Comment

  1. Shahaan Smith

    17 April, 2014 at 8:56 PM

    Hi Dr. Shevde,

    What alternative methods exist for reprogramming adult cells into iPS cells, excluding methods that introduce foreign genetic material into the host cells? If there are any, what are the major limitations of these methods?

    Thank you!

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