PSC Stress Management: Minimizing the Impact of Stress on Pluripotent Stem Cell Health in a Range of Workflows

By on March 2, 2015
Ask the Expert

Have you ever wished you had a safety net for your pluripotent stem cells, suffering loss of a cell line during processes such as cryopreservation, electroporation, or single cell passaging?

While stem cells have a tremendous proliferative capacity, long term culture of these cells has been shown to cause an accumulation of mutations that result in genetic instability, increasing tumorigenicity and thus limiting their usefulness in research and clinical applications. Solutions to reduce the stress on pluripotent stem cells provides multiple benefits including enhanced post-thaw recovery, as well as providing support during manipulation of cells in diverse workflows including high throughput screening and gene editing. Furthermore, these solutions provide greater consistency between experiments and operators. Discover solutions to help your cells stress less – visit this week’s Ask the Expert session and submit your questions.

This Ask the Expert session is sponsored by Thermo Fisher Scientific and hosted by Dr. Rhonda Newman. Dr. Newman joined Life Technologies in 2010 and is currently working on next generation stem cell culture and differentiation systems, enabling researchers to efficiently culture, expand, cryopreserve, and differentiate their stem cells to various cellular lineages. She received a Ph.D. at the University of Iowa from the lab of Dr. Madeline Shea, focusing on ligand induced allostery of calmodulin and its impact on regulation of the Ryanodine Receptor Type 1. Subsequently, she completed postdoctoral training in the lab of Dr. Ken Prehoda at the University of Oregon, studying the role of intramolecular interactions in regulating cell signaling cascades in the process of assymetric stem cell division

Don’t miss this chance to have your reprogramming questions answered today!

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