Improving Live Cell Fluorescence Imaging

By on June 15, 2015
Ask the Expert

Live cell fluorescence imaging provides the opportunity to study cellular function, however there can be challenges with the ability to image weak fluorophors without damaging the health of your cells.

During this Ask the Experts session, we will be discussing the challenges associated with imaging weak fluorophors without damaging the cells, photobleaching, or negatively impacting cell health and how Thermo Fisher Scientific has addressed this problem with a specialized media formulation, FluoroBrite DMEM™. FluoroBrite DMEM is a DMEM-based formulation with background fluorescence that is 90% lower than that emitted by standard phenol red-free DMEM. FluoroBrite DMEM has been designed to enhance the signal-to-noise ration of fluorophors so that researchers can visualize even the weakest fluorescent events in an environment that promotes optimum cell health.

Questions may include:

Questions may include topics such as how to improve the imaging of cells with culture media versus PBS and how you can both culture and image cells in the same media.

Who should visit the session?

  • Are you trying to fluorescently image a difficult to express protein?
  • Are you looking to increase the signal to noise ratio of your live cell imaging assays?
  • Are you changing from media to PBS for your imaging assays and losing precious cells?
  • Are you performing live cell imaging in PBS? Cells changing morphology when not in culture media?
  • When using phenol red-free media for imaging do you still see high background fluorescence?

If so, please take advantage of the opportunity to ask our expert a question and participate in a lively discussion on Live Cell Fluorescence Imaging.

This Ask the Expert session is sponsored by Thermo Fisher Scientific and hosted by Virginia Spencer. Dr. Spencer is a R&D scientist with a PhD in biochemistry and epigenetics. She completed a post-doc at Lawrence Berkeley National Lab and has extensive experience in live cell fluorescence imaging. Virginia has been working as an R&D scientist for ThermoFisher Scientific since 2010 and is currently developing products for improving cell culture performance and the live cell imaging experience.

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