How to Improve Protein Productivity Through Scale Up, Including Single-Use Bioreactors

By on March 7, 2016
Dr. Adam Elhofy, Ph.D., CSO.

Enhancing protein production is a common goal in the biomanufacturing industry. However, scalability is a problem faced across many platforms in manufacturing.  A desired outcome observed in shake flasks, such as increased protein productivity, is not always observed in large scale bioreactors.  Further, the move toward single-use bioreactors has posed an additional challenge, as productivity improvements observed in glass shake flasks or stainless steel bioreactors may not be observed in plastic bioreactor bags.  Availability of lipids in media is one factor to consider during bioproduction scale-up and when moving toward single-use system.

During this Ask the Expert session, we will be discussing considerations to be made regarding bioproduction scale-up and single-use bioreactors. Essential Pharmaceuticals is addressing these challenges with their Cell-Ess lipid supplement.  Cell-Ess was used as a feed at 5% (v/v) in a small Wave bioreactor resulting in greater than 25% monoclonal antibody titer increase, which was similar to the gains seen in earlier experiments using shake flasks.  Small bioreactors have been used as models to show scalability to up to 10,000-liter manufacturing runs.  These new data suggest supplementing with Cell-Ess as a feed will result in increased monoclonal antibody titers for 10,000-liter bioreactor runs, including in single-use bioreactors.

Who should visit?

  • Those in early development of bioproduction of proteins in eukaryotic cells
  • Those in media optimization or yield optimization for scaled bioproduction of proteins
  • Department Directors, Program Directors, Project leaders, involved in optimization of large scale bioproduction of proteins.

Types of Questions?

  • How does lipid availability in media influence protein productivity?
  • What can contribute to differences observed during the scale up process?
  • Why might lipid availability be different in single-use bioreactors?
  • What mitigations can improve lipid half-life and availability in media?
  • How can supplements be used in large scale bioproduction to improve protein productivity?

This Ask the Expert session is sponsored by Essential Pharmaceuticals and is hosted by Dr. Adam Elhofy, Ph.D., CSO. Dr. Elhofy developed the core technology for the Ess line of products and aided in creating patents around novel uses of materials.  Dr. Elhofy has over 14 years of scientific research experience in the areas of immunology, neuroscience, and oncology.  He was funded by both the National Institutes of Health and the Multiple Sclerosis society as an investigator at Northwestern University Medical School. His doctoral research won him the award of the Top 5 trainee scientists by the American Association of Immunologists. Dr. Elhofy has 14 scientific publications in peer reviewed journals. He has played a variety of roles with start-up biotech companies ranging from Principal Investigator to Director of Corporate Development.

For more information, please see “New Advances Pose New Challenges to Bioproduction.”

Please take advantage of the opportunity to ask our expert a question and participate in a lively discussion on animal origin free media optimization


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