We are adding simple fatty acids to our media, and we use plastic bioreactors. Are our simple fatty acids sticking to the plastic, or is it just complex fatty acids that are the problem?

Question

We are adding simple fatty acids to our media, and we use plastic bioreactors.  Are our simple fatty acids sticking to the plastic, or is it just complex fatty acids that are the problem?

Accepted Answer

Free fatty acids are added in some media optimization strategies.  Free fatty acids have a very short half-life once they are in solution.  Furthermore, all types of free fatty acids (both simple and complex) bind to vessel walls, especially in SUBs, where they will be mostly adhere to the vessel with 24 hours.

Answer

When would you add this is it a supplement or a feed?

Each time we add lipids to our media, we must make fresh solution and titrate the appropriate amount to add to avoid cell death. Do we have to undergo the same process with your supplement?

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