Ask the Expert – Media Optimization Can Improve Glycosylation Patterns and Consistency to Impact Protein Efficacy

By on November 28, 2016
Ask the Expert

When producing a biologic therapeutic, protein quality is a key consideration.  While manufacturing parameters such as high protein yield, protein solubility and protein stability are important, it is also critical that the biologic exhibits the desired therapeutic activity. Post-translational modifications impact efficacy and pharmacokinetics of the biotherapeutic, where improper glycosylation can result in an ineffective drug.  Proteins are commonly post-translationally modified by adding sugar residues in a process called glycosylation.  Half of all human proteins are estimated to be glycosylated.

During this Ask the Expert session, we will be discussing factors that impact glycosylation and tools available to improve the glycoform complexity and increase consistency of glycosylation profiles. Essential Pharmaceuticals is addressing these challenges with their Cell-Ess universal titer boost and optimizer.  Cell-Ess was used as a feed in a Wave bioreactor, resulting in greater than 25% increase in monoclonal antibody titer, which was similar to the titer increases observed in earlier experiments using shake flasks.  In addition to increasing titer, Cell-Ess was shown to significantly increase consistency of glycosylation patterns when used as a feed.  Further, Cell-Ess was shown to increase higher order glycoforms measured by increase in galactosylation.  These new data suggest supplementing with Cell-Ess as a feed increases protein titer while improving or maintaining quality.

Who should visit?

  • Those in process development for bioproduction of proteins in mammalian cells such as CHO cells
  • Those in media optimization or yield optimization for scaled bioproduction of proteins
  • Department Directors, Program Directors, Project leaders, involved in optimization of large scale bioproduction of proteins.

Types of Questions?

  • What factors influence glycosylation patterns?
  • What impact does glycosylation have on efficacy?
  • What factors influence consistency of glycosylation patterns from run-to-run?
  • What are the benefits of the higher order glycoforms?

This Ask the Expert session is sponsored by Essential Pharmaceuticals and is hosted by Dr. Adam Elhofy, Ph.D., CSO. Dr. Elhofy developed the core technology for the Ess line of products and aided in creating patents around novel uses of materials.  Dr. Elhofy has over 14 years of scientific research experience in the areas of immunology, neuroscience, and oncology.  He was funded by both the National Institutes of Health and the Multiple Sclerosis society as an investigator at Northwestern University Medical School. His doctoral research won him the award of the Top 5 trainee scientists by the American Association of Immunologists. Dr. Elhofy has 15 scientific publications in peer reviewed journals. He has played a variety of roles with start-up biotech companies ranging from Principal Investigator to Director of Corporate Development.

For more information, please see “Cool Tool – Novel universal titer boost and enhancer improves CHO cell protein production in small bioreactors.”

Please take advantage of the opportunity to ask our expert a question and participate in a lively discussion on animal origin free media optimization.

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