- Cool Tool – An Optimized, Chemically-Defined, Animal Component-Free Neural Basal MediumPosted 2 days ago
- Cool Tool – Lynx CDR Connectors to Improve Sterile Fluid Transfer in BiomanufacturingPosted 3 days ago
- Improving Glycosylation Patterns and Consistency Through Media OptimizationPosted 5 days ago
- Cool Tool – Online Cell Culture Media Formulation ToolPosted 1 week ago
- Video – Impact of Chemically Defined Media on Product QualityPosted 2 weeks ago
- Ask the Expert – Media Optimization Can Improve Glycosylation Patterns and Consistency to Impact Protein EfficacyPosted 2 weeks ago
- Digital Biomanufacturing Will Enable Tissue BioprintingPosted 3 weeks ago
- Video – When and Where to Optimize Cell Culture MediaPosted 4 weeks ago
- Cool Tool – SCOUT® technology reduces time to market and increases chance of success for biopharmaceutical productsPosted 4 weeks ago
- Pumping Iron – But Not in the gym: The Critical Roles of Transferrin in Cell Culture MediaPosted 1 month ago
Got mRNA? Solve DNA transfection issues with mRNA transfection.
Typically, DNA enters the nucleus when cells divide because cell division creates small nuclear pores. In non-dividing primary cells DNA doesn’t enter the nucleus making these cells very hard-to-transfect. If DNA entry is a bottleneck, why not deliver mRNA directly?
Please reach out and ask our expert your transfection questions! Nektaria is here to help answer any questions. What’s the best way to transfect mRNA? Which reagent should I use? Can I transfect primary cells with a reagent? How do I prepare an mRNA template from DNA? What’s the protocol? These are just some examples of the type of questions to be covered in this Ask the Expert Session.
This Ask the Expert Session is Sponsored by Life Technologies and hosted by Nektaria Andronikou. Nektaria joined Life Technologies in 2010 and is currently working with the transfection team on the development of new delivery methods targeting relevant cellular models that will enable the use of exciting new technologies. She received a Bachelor of Science in Biochemistry with a minor in Cellular and Molecular Biology from UCSD. She began her professional career at ISIS Pharmaceuticals, as a research associate for the Cardiovascular Drug Discovery program, screening numerous pre-clinical targets that led to the discovery of the now FDA approved antisense drug, Kynamro.