Transfection and Selection

Sponsored by: Life Technologies
Session ends: November 8th, 2013, 3:00pm MST
Answers by: Timothy Fawcett, Ph.D., Director, BioTechnical Institute of Maryland (BTI)

Introduction

Transfection is often used to transfer an expression plasmid into an animal cell with protein expression as the goal.  There are multiple methods to accomplish this and many opportunities to optimize the process.  Do you know the best growth conditions for your cells when they are going to be transfected?  If you wanted to isolate clones during selection would you know the best way?  This session is a great opportunity to ask your questions about transfection and selection and practical, applicable answers.

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Questions & Answers

I am at the point where I have to transfect my cells and I read about both electroporation and chemical transfections. I have a fairly large plasmid (20Kb) and I wanted your opinion about the best way to transfect my cells.

Great question to start this “ask the expert”. Yes both electroporation and chemical transfection are the two most popular methods for moving DNA into cells. The advantage of electroporation is that you can get DNA into all cell types and it is especially good for eukaryotic cells and stem cells. It works by using a […]» Read More