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Yes, you can thaw Corning® Matrigel® matrix overnight in a refrigerator. First submerge Matrigel matrix vials in an ice bucket filled with ice at 2°C to 8°C. Place the covered ice bucket toward the back of the refrigerator where it will not be subjected to temperature change. Use an adequate amount of ice so that the Matrigel matrix vial is in ice for the entire thawing process (not in cold water). Once thawed, swirl the vials in ice to ensure the material is evenly distributed.