Do i need to inject a 100% pure antigen in order to get a desired results or can I use a 80% purity antigen for injection, as the antigen I am dealing with will only grow along with the bacteria. I’m also using non spf female balb/c mices for the injection. I’m wondering if all these factors may affect to get the desired results.

Answer

About your antigen, if you were making polyclonal antibodies the purity would be a problem. Technically, by making monoclonal antibodies you can still get the one you want. But I do envision some problems. Obviously your search for the MAb you want will be made more difficult due to the 20% non-antigen directed Abs. The major problem will be with the search itself. I am not sure how you will find your specific MAbs if you don’t at least have pure epitope to facilitate your search. I would suggest that after you do the fusion, subtract the hybridoma cells that are producing MAb against the bacteria as a first screen by using pure bacteria without your antigen. This will help speed things in your search.