I am looking for the most inexpensive way to culture hybridoma cells in serum free conditions. I am starting with classic media, is there anything that is serum free that I can add to classic media to allow for serum free culture or do I need to use a pre-made serum free medium for hybridoma cells.


Moving cells from serum-containing to serum-free media is not so easy since adaptation is required. To my knowledge there are no additives such as a non-animal derived substitute that can be added to a basel (classic) medium that will make it perform like a serum containing medium. For your information there are media such as D-MEM or RPMI-1640 which are used for hybridoma culture, that have modified formulations (Advanced-DMEM or Advanced-RPMI1640 from GIbco for example) that work very well for hybridoma culture using only 2% FBS this could reduce your cost somewhat without losses in performance. SInce you asked specifically about the expense of hybridoma culturing…I think about several issues when I think about cost. First, a bottle of D-MEM supplemented with 10% FBS might cost $40-$50 (currently the cost of serum is low) compared to a serum-free medium such as Gibcos PFHM II, Hybridoma-SFM or CD-Hybridoma which might range from $70-$80. (If the cost of serum gets higher then the price gap decreases further). The cost increase for using a serum-free media is in my mind, is a small price to pay for the many resulting benefits. Second, all of the serum-free media designed for hybridoma culture out-perform when compared to classic media in terms of cell growth, viability and MAb production. third, using a serum-free medium make for much easier downstream purification of your product and finally if you are making your MAbs for diagnostic or clinical work the regulatory hurdles may be simplified.

Pin It on Pinterest