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There are numerous options for high-throughput screening spheroids. The right option will be dependent on what you want to measure and the instrument available to you. A good homogenous method for assessing viability is using Promega’s CellTiter-Glo 3D reagent to measure ATP. It’s important to use the 3D version of the reagent since spheroids typically require stronger lytic reagents than what is required for a 2D monolayer assay. It’s also possible to use high throughput imagers via phase or fluorescent microscopy and image analysis software to quantify changes in spheroid size, metabolic activity and fluorescent staining.
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