I am exploring some high-throughput options for screening spheroids. Do you have any suggestions?

Answer

There are numerous options for high-throughput screening spheroids. The right option will be dependent on what you want to measure and the instrument available to you. A good homogenous method for assessing viability is using Promega’s CellTiter-Glo 3D reagent to measure ATP. It’s important to use the 3D version of the reagent since spheroids typically require stronger lytic reagents than what is required for a 2D monolayer assay. It’s also possible to use high throughput imagers via phase or fluorescent microscopy and image analysis software to quantify changes in spheroid size, metabolic activity and fluorescent staining.

Please find some useful links below:

Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read-outs

High-Throughput Confocal Imaging of 3D Spheroids

CAR-T Cell Screening in Tumor Spheroids Using Corning Spheroid Microplates

Spheroid Formation Protocol