Unlocking the complexities of cancer is an ongoing challenge. Luckily, a countless number of researchers are working every day – determined to understand the causes and behaviors of cancer in search of new and better ways to more effectively treat the disease.
Corning Life Sciences supports this important research with reliable lab products that deliver consistent, repeatable results. Matrigel® matrix, Transwell® permeable supports and spheroid microplates are just a few Corning products being used to create more in vivo-like 3D environments used in cutting edge cancer research studies.
This week Corning experts will be answering your questions on cancer research – our scientists are ready to support you in areas ranging from 3D cell culture to tumor formation and angiogenesis to invasion/migration assays
About Our Experts
Corning experts include Paula Flaherty, Technology Manager, Jeffrey Partridge, Senior Development Scientist, Hilary Sherman and Audrey Bergeron, both Applications Scientists. Paula has extensive experience in developing cell based assays. Jeff has over 20 years’ experience in cancer research, including using animal models as well as in vitro invasion and migration systems such as Corning® BioCoat™ Matrigel® Invasion Chambers and Corning FluoroBlok™ Cell Culture Inserts. Hilary has extensive experience with cancer cell migration, immune oncology assays and 3D cell culture. Audrey has experience with cultures and applications of primary and engineered immune cells as well as with high throughput 2D and 3D assays.
There is a lot of expectation about modeling cancer organoids but is there an application of Corning Fluoroblok inserts to track immune therapy with an organoid culturing into Corning Biocoat Matrigel Invasion Chambers? In other words, can I culture an organoid in the inner compartment and then tracking the effect of the immune therapy?
Assuming you are looking to culture a cancer organoid in the apical chamber of Corning® FluoroBlok™ Cell Culture Inserts and measure immune function, then the answer is most likely. Although it does not appear much published work has been done in this area, FluoroBlok inserts are a great choice when one is looking to exclude … Continued
I am looking for a reliable transfection protocol for DU 145 prostate cancer research. Any recommendations?
Although Corning does not have any DU145 specific transfection protocols, we do know that DU145 as well as many other prostate lines behave differently in 3D as opposed to 2D. 3D transfection is possible in Corning® Matrigel® matrix, as well as in Corning Ultra Low Attachment vessels and Corning spheroid microplates. There are several protocols … Continued
There are numerous options for high-throughput screening spheroids. The right option will be dependent on what you want to measure and the instrument available to you. A good homogenous method for assessing viability is using Promega’s CellTiter-Glo 3D reagent to measure ATP. It’s important to use the 3D version of the reagent since spheroids typically … Continued
I don’t have a specific question but was wondering if you could tell us about which promising new cancer research tools you think will make the most impact.
There are many exciting things happening in the cancer research space, but two areas that we feel will have the largest impact on cancer research in the next few years are through improved modeling and superior imaging. An interesting trend we have noticed, is that the technologies are not necessarily all new, but have been … Continued
I am interested in learning more about laminins, I think you offer these, and which types of culture they can be beneficial in.
There are several different formats of laminin available. Here is an overview of what Corning offers, whether vialed (murine), coated, or recombinant (human). Vialed products (mouse) Corning® Laminin (mouse) is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It … Continued
What do you think is the most successful ECM, culture vessel combination for culturing colon cancer spheroids?
A great option for this type of work would be ultra-low attachment plates like the Corning® spheroid microplate as an optimal cell culture vessel. They are easy to use, promote formation of one organoid per well of uniform size and allow for fluorescent, luminescent and imaging assays to be conducted right in the culture plate. … Continued
Advice on the most efficient way to differentiate between live and dead cells when using flow cytometry?
Hoechst is great for counting all cells since it stains all nuclei (live and dead cells). To enumerate dead cells we routinely use Propidium iodide (PI). PI is a membrane impermeable dye that is excluded form viable cell membranes. If your cells are positive for PI they are most likely dead or apoptotic. Both stains … Continued
I am looking for an efficient and easy way to isolate B cells from whole blood without using magnetic beads. Any suggestions?
The first step in B cell isolation is to isolate mononuclear cells from whole blood through centrifugation with a Ficoll gradient. Here is a protocol for reference that uses Corning Lymphocyte Separation Medium as part of this process. There are many different B cell isolation kits that are commercially available, however, you will find it … Continued
At ISSCR this year, I went to a talk on co-culturing tumor spheroids and immune cells to create cancer models. I have also seen fibroblasts used in the same way. Do you have any resources to learn more about creating this type of model. I have seen papers on various models, but I am looking for resources for how to create these in the lab.
One of the simplest methods to create co-culture spheroids containing multiple cell types is to seed the different cell types together into a low attachment round bottom plate such as the Corning® spheroid microplate. The cells can be seeded at the same time or added at different time points. Here is an application note as … Continued