Hi, I am wondering if the CHO genomic sequence known?
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This is a good question and the answer is yes! The paper by Xun Xu et al., Nature Biotechnology, Vol 29, No 8, 201, pg 735. reports that a CHO-K1 ancestral cel line was sequenced. There were 24,383 predicted genes. What is very interesting about the paper is that the authors focused on investigating genes involved in glycosylation. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome. The authors also point out correctly that this is important since CHO cells are used to make human like therapeutic antibodies and glycosylation patterns are critical to function and turnover rates. Ultimately this research may lead to making CHO cells even better for bio-production than it already is.
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