How can I spot mycoplasma contamination?

Question

Accepted Answer

Mycoplasma contamination is indeed a key factor to evaluate during a cell culture projects, as it can influence cell behavior, morphology and function. Moreover, you may be asked for mycoplasma testing results by journal article reviewers (example: https://www.nature.com/nature-research/editorial-policies/reporting-standards). Fortunately, there are simple fluorochrome DNA staining tests, as well as PCR based mycoplasma tests, that can detect mycoplasma. These can be done simply in your lab, or your samples can be sent out to an outside lab for testing.

Answer

In our group we always use aseptic techniques and fresh media but we still have bacterial contamination, with media turbidity and pH decrease. Where can it come from?

We have a protocol for freezing cells in our lab, but we have some contamination. Do you have a checklist you can recommend to reduce the possibility of contamination in frozen cell stocks.

Pin It on Pinterest