In our group we always use aseptic techniques and fresh media but we still have bacterial contamination, with media turbidity and pH decrease. Where can it come from? 


Often cabinets and incubators can be a source of contamination since these are often shared among multiple people/research groups. It is therefore important to have regular maintenance and cleaning of these. Incubators should be cleaned with Lysol and 70% EtOH once a month and shelves should be autoclaved. Clean water tray often (autoclaved, distilled water) and clean spills immediately. Daily cleaning of hood space with 70% EtOH, as well as monthly cleaning with 10% bleach or equivalent products is suggested.

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