How can I spot mycoplasma contamination?
This question is part of the following Ask The Expert session:
How to minimize contamination risk and protect your cultures
Job Title: Scientific Supporting Specialist supporting EMEA regions
Mycoplasma contamination is indeed a key factor to evaluate during a cell culture projects, as it can influence cell behavior, morphology and function. Moreover, you may be asked for mycoplasma testing results by journal article reviewers (example: https://www.nature.com/nature-research/editorial-policies/reporting-standards). Fortunately, there are simple fluorochrome DNA staining tests, as well as PCR based mycoplasma tests, that can detect mycoplasma. These can be done simply in your lab, or your samples can be sent out to an outside lab for testing.